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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: J Immunol. 2010 Apr 30;184(11):6067–6075. doi: 10.4049/jimmunol.0901618

Figure 6.

Figure 6

Effects of age on antibody responses to the “neo-self” Talpha antigen. Young (5 months of age) and old (23-27 months of age) mice were immunized twice at one month intervals with 10 μg of Talpha (aa1-210) as described in Methods. Blood samples were taken one week after the secondary immunization, serum was prepared, and the antibody titer to Talpha (aa1-210) was measured in serum dilutions by ELISA. GraphPad Prism 5 was used to fit the titer points from each mouse to a sigmoidal curve and to determine the reciprocal dilution of serum required to obtain an absorbance of 0.1 O.D. above background.