Figure 2.

Deletion of HDAC1&2 increases spontaneous excitatory neurotransmission in immature hippocampal neurons. A, A schematic of the experimental timeline showing that young neurons at 2 DIV were infected with lentivirus (GFP or CRE) and recordings were performed on 7–8 DIV. B, E, H, Representative recordings of miniature excitatory events recorded in 1 μm tetrodotoxin and 50 μm picrotoxin in GFP- and CRE-infected HDAC1, HDAC2, or HDAC1&2 neurons, respectively. C, F, I, Bar graph depicting the mEPSC frequency following the loss of HDAC1, HDAC2, or HDAC1&2. The loss of HDAC1 or HDAC2 had no significant effect on mEPSC frequency, while the loss of HDAC1&2 produced a significant increase in mEPSC frequency. D, G, J, Bar graphs of mEPSC amplitudes showing no change following the loss of HDAC1, HDAC2, or HDAC1&2 in young neurons.