Figure 5.

HDAC1&2 deletion using low-titer lentiviral infection does not affect spontaneous excitatory neurotransmission. A, Schematic timeline of the experiment showing that 2 DIV after floxed HDAC1&2 neurons were plated and infected with low titer lentivirus expressing either GFP or CRE. B, Representative recordings of miniature excitatory events in GFP- and CRE-infected neurons (low-titer) in 1 μm tetrodotoxin and 50 μm picrotoxin. C, Bar graph showing that mEPSC frequency was not significantly different when recorded from an infected neuron in which the majority of presynaptic cells were uninfected. D, Bar graph of mEPSC amplitudes, also showing no change following the low-titer lentiviral infection of floxed HDAC1&2 neurons.