Figure 7.

Overexpression of HDAC2 increases spontaneous excitatory neurotransmission in mature hippocampal neurons A, Schematic of the experimental timeline showing that hippocampal neurons were infected with lentivirus expressing either GFP or GFP-HDAC2 on 7 DIV and then recorded at 16–18 DIV. B, Representative recordings of miniature excitatory events in GFP and GFP-HDAC2-infected neurons. Recordings were made in Tyrode's solution containing 1 μm tetrodotoxin and 50 μm picrotoxin. C, Bar graph depicting the significant increase in mEPSC frequency observed by HDAC2 overexpression. D, A bar graph of mEPSC amplitude did not reveal a significant change in amplitudes between GFP- and GFP-HDAC2-infected mature neurons.