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. Author manuscript; available in PMC: 2010 Jul 2.
Published in final edited form as: Nat Cell Biol. 2009 Jun 14;11(7):832–838. doi: 10.1038/ncb1890

Figure 1. Chromosomes can align in cells lacking K-fibres.

Figure 1

(a) HeLa cells transfected with luciferase control RNAi oligo (Control), hNuf2 RNAi oligo, or hNuf2+HSET RNAi oligo and stained to visualise HSET (green) Hec1 (red), and DNA (blue). Scale bar, 10 µm. (b) The average percentage of prometaphase and metaphase cells with aligned chromosomes is graphed ± s.d. from three independent experiments where ~100 mitotic cells were scored per experiment. The differences between all the pairs are significant (p<0.05) (c) Western blot analysis of samples from each knockdown probed with antibodies to HSET, Hec1, and tubulin.