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. Author manuscript; available in PMC: 2010 Jul 2.
Published in final edited form as: Nat Cell Biol. 2009 Jun 14;11(7):832–838. doi: 10.1038/ncb1890

Figure 3. In the absence of K-fibres, kinetochores congress with alternating kinetochores leading.

Figure 3

Triple labelled HeLa cells (GFP-CENP-A, GFP-gamma-tubulin, and mcherry-H2B) treated with control luciferase (a) or hNuf2+HSET (c) RNAi were imaged at 30 s interval from nuclear envelope breakdown until telophase and analysed for kinetochore orientation during congression. The time stamp is marked in the upper left of each panel, asterisks mark the position of spindle poles, and the coloured arrowheads mark individual kinetochore pairs schematised in (b) and (d). Scale bar, 10 µm. Note that the arrow heads that reflect the orientation of the kinetochore pairs in the control cells do not change much over time, whereas the arrow heads in the hNuf2+HSET RNAi cell change orientation dramatically with time.