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. Author manuscript; available in PMC: 2010 Jul 2.
Published in final edited form as: Nat Cell Biol. 2009 Jun 14;11(7):832–838. doi: 10.1038/ncb1890

Figure 4. Chromosome congression in cells with disrupted K-fibres is dependent on CENP-E.

Figure 4

(a) Cells in which hNuf2, HSET, and CENP-E were knocked down were stained to visualize HSET (green), Hec1 (red) and DNA (blue). Scale bar, 10 µm. (b) Quantification of the percentage of bipolar spindles with aligned chromosomes from the four different RNAi conditions. The mean percentages of spindles ± s.d. were graphed for each condition. Three independent experiments and approximately 300 total cells were scored per RNAi condition. The differences between the mean percentages of control versus hNuf2 RNAi, hNuf2 RNAi versus hNuf2+HSET RNAi and hNuf2+HSET versus hNuf2+HSET+CENP-E RNAi are all significant (p<0.05). The difference between hNuf2 RNAi and hNuf2+HSET+CENP-E RNAi is not significant (p=0.45).