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. Author manuscript; available in PMC: 2010 Jul 2.
Published in final edited form as: Stem Cells. 2009 Apr;27(4):806–811. doi: 10.1002/stem.31

Figure 2. The directed differentiation of hESC and human iPS-derived cells recapitulates the stereotypical progression associated with motor neuron formation.

Figure 2

After 8–10 more days in the presence of RA, Shh pathway agonists and neurotrophic factors, both HSF1 and human iPS-derived EBs contained Sox3+, Nkx6.1+ and Olig2+ motor neuron progenitors (a, b, d, e). Both HSF1 and human iPS cells were further able to produce differentiated Islet1 and βIII-tubulin-positive motor neurons within these EBs (c and f). Scale bar: a–f, 100μm.