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Degenerate primers targeting minimal Type II PKS genes were used to identify KSβ sequences present in unique eDNA library aliquots constructed from soil samples collected in Utah (A) and California (B). ClustalW48 derived phylogenetic trees of the KSβ sequences identified in these screens are shown. The aliquots from which sequences were amplified and the point at which they began to reappear in the library (red) are shown as a heatmap.
