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. 2010 Sep;93(9):833–844. doi: 10.1002/bip.21450

FIGURE 4.

FIGURE 4

(A) pTARa contains elements that allow for the rapid assembly and propagation of pathways in S. cerevisiae (green), the transformation and analysis of these pathways in E. coli (blue) and the integrative conjugation of assembled pathways into Streptomyces (yellow). For capture vector construction, pathway-specific upstream (US-blue), and downstream (DS-red) homology arms, as well as a counter selection cassette (cyh/bla) are incorporated into the capture vector.23, 24 During recombination, the counter selection cassette is exchanged for a TAR cloned gene cluster (B) or TAR reassembled eDNA pathway (C).