FIGURE 5.

(A) We used pTARa to directly and specifically clone the colibactin gene cluster from C. koseri genomic DNA. Predicted HindIII cut sites and restriction fragment sizes are marked on the map of the pTARa-Colibactin construct. The size of the gene cluster is listed. (B) The experimentally determined HindIII restriction map of pTARa-Colibactin is shown. Two images of the same digest were taken at different points during electrophoresis to highlight fragment sizes more clearly (M = Lambda HindIII digest, M* = 50 bp ladder). (C) TAR cloning of gene clusters circumvents the need to construct and screen a genomic library.