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. Author manuscript; available in PMC: 2010 Jul 2.
Published in final edited form as: Nucleosides Nucleotides Nucleic Acids. 2008 Jun;27(6):608–619. doi: 10.1080/15257770802138558

FIGURE 1.

FIGURE 1

Uric acid induces production of reactive oxygen species in mouse adipocytes. Differentiated mouse adipocytes were treated with 15 mg/ml uric acid for 30 minutes with or wirhout 25 μM Mn(II) tetrakis(1-methyl-4-pyridyl)porphyrin (MnTMPyP), N-acetyl cysteine (10 mM), apocynin (200 μM) and diphenylene-iodonium (DPI, 10 μM), rotenone (100 μM), and thenoyltrifluoroacetone (TTFA, 100 μM) followed by the measurement of ROS with the ROS-specific fluorescent probe 5 (and 6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate-acetyl ester (CM-H2DCFDA) as described in details in (33). (A) Effect of uric acid is blocked by the superoxide scavenger MnTMPyP; (B) Effect of uric acid is blocked by inhibitors of NADPH oxidase apocynin and DPI but not the inhibitors of mitochondrial respiratory chain rotenone and TTPA. *— the effect of uric acid is significant (P < 0.05, nonparametric U-test); & —the effect of the inhibitor is significant (P < 0.05, U-test, n = 3). (Reproduced from (33)—used with the permission from the American Physiological Society)