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. Author manuscript; available in PMC: 2010 Jul 2.
Published in final edited form as: Nucleosides Nucleotides Nucleic Acids. 2008 Jun;27(6):608–619. doi: 10.1080/15257770802138558

FIGURE 4.

FIGURE 4

Uric acid induces NADPH oxidase-dependent lipid oxidation in mouse adipocytes. Adipocytes were treated with 15 mg/ml uric acid with or without superoxide scavenger MnTMPyP and an inhibitor of NADPH oxidase apocynin as indicated in Figure 1. Cells were loaded with the oxidation-sensitive lipid probe C11-BODIPY581,591 followed by washing cells to remove unincorporated probe. Detection of lipid peroxidation by live imaging was started immediately after addition of uric acid. (A) Green fluorescence representing oxidized probe was detected every 60 seconds for 100 ms after addition uric acid. (B) Ratiometric analysis of lipid oxidation: ratio of green/red fluorescence measured every 60 seconds is shown. The values for 12–20 different cells (mean ± SD) are shown.