Skip to main content
. Author manuscript; available in PMC: 2010 Jul 2.
Published in final edited form as: Adv Enzyme Regul. 2009;49(1):75–86. doi: 10.1016/j.advenzreg.2009.01.006

Figure 2.

Figure 2

Domain and primary structure of Sac1 protein family. A Diagrams of the domain organization of Sac1 proteins. Domains and motifs of interest are highlighted by colored boxes. Blue boxes show the Sac1 catalytic domain, yellow boxes show the CX5R(T/S) core catalytic motif, green boxes show the two transmembrane domains (TMD I and TMD II respectively), and the orange boxes show the KXKXX COPI binding motif. Three key amino acids within the Sac1 domain known to affect catalytic activity (Rivas et al., 1999; Liu et al., 2008) are indicated in red. B Sequence alignment of the core catalytic domains and the KXKXX COPI binding motifs of the Sac1 protein family. The CX5R(T/S) core catalytic domain of Sac1 is highly conserved in yeast, higher plants, Drosophila, and mammals, whereas the KXKXX COPI binding motif is unique to mammalian Sac1 proteins. Identical amino acids are highlighted by black boxes. C. Phylogenetic tree of the Sac1 protein family.