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. Author manuscript; available in PMC: 2011 Jun 8.
Published in final edited form as: Biochemistry. 2010 Jun 8;49(22):4611–4619. doi: 10.1021/bi902083n

Fig. 5. P2X7 is glycosylated on N187, N202, N213, N241 and N284.

Fig. 5

A) Wild-type P2X7 (WT) and the Asn to Ala mutants were expressed in HEK293 cells and cell lysates were immunoblotted with anti-Myc antibody. The five single Asn to Ala mutants exhibited faster electrophoretic mobility than WT receptor, and the double mutants migrated further than the single mutants. The proteins were denatured and treated with B) EndoH or C) PNGase F for ~1 h at 37°C and immunoblottred with anti-Myc antibody. The data shown in panels A-C are representative of two or more experiments. D) P2X7 WT and Asn to Ala mutants were expressed in HEK293 cells and lysates were immunoblotted with anti-Myc. The P2X7 N187A mutant does not migrate as fast as the other Asn to Ala mutants but does migrate faster than WT. These data are representative of >10 experiments.