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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Nat Immunol. 2010 Apr 25;11(6):520–526. doi: 10.1038/ni.1866

Figure 3. STAT6 phosphorylation occurs in draining but not non-draining lymphoid tissues.

Figure 3

(a) huCD2 expression in 4get/KN2 IL-4 dual reporter mice infected with H. polygyrus. Graphs indicate percentage of huCD2+ cells within the total lymphocyte population. (b) STAT6 phosphorylation in 4get mice 2 weeks post H. polygyrus infection. Horizontal lines indicate mode fluorescence intensity of p-STAT6 staining in the negative control (mesLN cells of Il4ra−/− mice). Graphs depict geometric mean fluorescence intensity of p-STAT6 in CD4+ GFP− cells. (c) huCD2 expression (gated on CD4+ cells) and (d) STAT6 phosphorylation (gated on CD4+ GFP− cells) in wild-type or Il4ra−/− 4get/KN2 mice infected with H. polygyrus and injected i.v. with either PBS or an anti-CD3ε antibody 2 weeks later. Graphs depict percentage of huCD2+ cells within the GFP+ CD4+ population. (e) STAT6 phosphorylation in 4get mice infected with H. polygyrus and injected i.v. with recombinant murine IL-4 on day 14. Data shown are gated on CD4+ GFP− cells and Il4ra−/− mice given IL-4 were included as a baseline staining control. In all panels, each data point represents an individual mouse and data are representative two or more independent experiments with 3–4 mice per group.