FIGURE 2.
(A) Sesamin suppresses the expression of TNF-induced tumor cell survival proteins. KBM-5 cells were incubated with 100 μM sesamin for 12 h and then treated with 1 nM TNF for the indicated times. Whole cell extracts were prepared and analyzed by Western blotting using the indicated antibodies. (B and C) Sesamin inhibits the expression of tumor cell proliferative and metastatic proteins. KBM-5 cells were incubated with 100 μM sesamin for 12 h and then treated with 1 nM TNF for the indicated times. Whole cell extracts were prepared and analyzed by Western blotting using the indicated antibodies. Anti-β-actin antibody was used as a loading control. Densitometric values of bands were corrected based on β-actin and were expressed relative to that of untreated cells, which was set as 1.0.