FIGURE 5.

(A) Sesamin inhibits TNF-induced nuclear translocation of p65 assayed by immunocytochemical analysis. KBM-5 cells were initially treated with 100 μM sesamin for 12 hr and then exposed to 0.1 nM TNF for 15 min. After cytospin, immunocytochemical analysis was done as described under “Materials and Methods.” (B) Sesamin inhibits TNF-induced phosphorylation of p65. KBM-5 cells were either untreated or pretreated with 100 μM sesamin for 12 hr and then treated with 0.1 nM TNF for the indicated times. Nuclear extracts were prepared and analyzed by Western blotting using p65 and phospho-specific p65 antibodies. For loading control of nuclear extracts, the membrane was reblotted with anti-PARP antibody, respectively.