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. Author manuscript; available in PMC: 2011 Jun 4.
Published in final edited form as: DNA Repair (Amst). 2010 Apr 21;9(6):678–689. doi: 10.1016/j.dnarep.2010.03.005

Figure 6.

Figure 6

Low doses of CPT reduce the frequency of spontaneous common fragile site breaks in ATR-deficient cells. (A) Western blot showing depletion of ATR after ATRflox/− cells are infected with adenovirus AdCre1. (B) Average spontaneous total chromosomal gaps and breaks per cell in ATRflox/− cells after 18 h in the presence of 0 to 100 pM CPT; n= 80 metaphases for each data set. Error bars indicate the 95% confidence interval. (C) Frequency (%) of spontaneous gaps and breaks at specific fragile sites FRA3B and FRA16D in ATRflox/− cells after 18 h in the presence of 0 to 100 pM CPT; n=51–75 sites examined. Fragile sites were identified by FISH. Frequency of fragile-site induction is presented as the percentage of chromosome 3 or 16 homologs with breaks at FRA3B (white) or FRA16D (gray), respectively. Error bars indicate the 95% confidence interval.