Table 1.
List of all chromosome bands and associated fragile sites with breaks in UML-49 cells after treatment with 0.4 μM APH, 30 nM CPT, or both.
| G band | APH (0.4uM)a | CPT (30nM)a | APH + CPTa | Associated Fragile Site |
|---|---|---|---|---|
| 3p14.2 | 24/96 | 1/96 | 2/96 | FRA3Bc |
| 16q23.2 | 32/96 | 0/96 | 5/96 | FRA16Dc |
| 7q31.2 | 5/96 | 0/96 | 3/96 | FRA7Gc |
| 7q32.3 | 0/96 | 0/96 | 1/96 | FRA7Hc |
| Xp22.3 | 1/96 | 0/96 | 0/96 | FRAXBc |
| 1p36.1-36.3 | 0/96 | 0/96 | 1/96 | FRA1A |
| 1p22.1-22.3 | 1/96 | 0/96 | 0/96 | FRA1D |
| 2q21.3 | 1/96 | 0/96 | 0/96 | FRA2F |
| 2q31 | 0/96 | 0/96 | 1/96 | FRA2Gc |
| 2q37.3 | 1/96 | 0/96 | 0/96 | FRA2J |
| 3q25.1-25.3 | 1/96 | 0/96 | 0/96 | FRA3D |
| 4q31.1 | 0/96 | 0/96 | 1/96 | FRA4C |
| 6q13 | 1/96 | 0/96 | 0/96 | FRA6D |
| 6q21 | 0/96 | 1/96 | 0/96 | FRA6Fc |
| 8q22.1 | 0/96 | 1/96 | 0/96 | FRA8B |
| 10q25.2 | 0/96 | 1/96 | 0/96 | FRA10E |
| 10q11.2 | 0/96 | 0/96 | 1/96 | FRA10G |
| 11p15.1 | 0/96 | 0/96 | 1/96 | FRA11C |
| 13q32 | 1/96 | 0/96 | 0/96 | FRA13D |
| 19q13.1-13.3 | 0/96 | 1/96 | 0/96 | FRA19A |
| 1p13.1-13.3 | 0/96 | 0/96 | 1/96 | - |
| 1q31 | 0/96 | 1/96 | 0/96 | - |
| 2p23 | 0/96 | 1/96 | 0/96 | - |
| 2q23 | 0/96 | 0/96 | 1/96 | - |
| 2q31 | 0/96 | 0/96 | 1/96 | - |
| 3p25.1-25.3 | 0/96 | 1/96 | 0/96 | - |
| 4q31.1-31.3 | 0/96 | 1/96 | 0/96 | - |
| 5q33.1-33.3 | 0/96 | 0/96 | 1/96 | - |
| 7p11.2 | 0/96 | 1/96 | 0/96 | - |
| 8q23 | 0/96 | 1/96 | 0/96 | - |
| 10p11.2 | 0/96 | 0/96 | 1/96 | - |
| 10q11.2 | 0/96 | 0/96 | 1/96 | - |
| 12p12.1-12.3 | 0/96 | 0/96 | 1/96 | - |
| 13q14.1-14.3 | 0/96 | 1/96 | 0/96 | - |
| Total # of breaks | 68 | 12 | 23 | |
| % of breaks at fragile sitesb | 93% | 25% | 57% | |
| % of breaks at non-fragile sites | 0% | 75% | 48% | |
a
Number of breaks at indicated band seen in 96 chromosomes.
b
Percentage of total breaks occurring at the 32 fragile sites that account for 93% of all gaps and breaks in human lymphocytes.
c
Fragile sites whose breakpoints have been defined by FISH.