Fig. (2).
In vitro differentiation of adipose derived SVCs. (a) Phase-contrast microscope image of cultured SVCs after 5 (top), 10 (middle), and 15 (bottom) days of in vitro differentiation. (b) Expression profile of proteins identified in differentiated SVCs showing members of the cardiac thin filament. (c) Calcium transient measurements and contractile performance in differentiated SVCs during field stimulation. (d) Differentiation potential assessed during manipulation with recombinant Wnt proteins. (e) Identification of Sca1+ and c-kit+ stem cell markers associated with adipose-derived SVCs. Figure has been adapted with permission from the Journal of Molecular and Cellular Cardiology [19]. Abbreviations: SVCs: stromal vascular cells; T.A.: tibialis anterior; Dkk1: dickkopf homolog 1.