. Author manuscript; available in PMC: 2011 Jun 1.

Published in final edited form as: Cancer Prev Res (Phila). 2010 May 25;3(6):727–737. doi: 10.1158/1940-6207.CAPR-09-0260

Table 1.

Primer sequences used for quantitative real-time PCR.

Gene	Forward (5’-3’)	Reverse (5’-3’)
17β HSD	CTTTATCCTGATTCGGGAACTG	GTCCTCAAGACTGAAGTTAGA C
CYP1A1	TGGAGACCTTCCGACATTCAT	GGGATATAGAAG CCATTCAGACTT G
CYP1B1	AACCCAGAGGACTTTGATCCG	CGTCGTTTGCCCACTGAAAA
COMT	GGATGCAGTGATTCGGGAGTA	GCAGCGTAGTCAGGGTTCATCT
GSTA1	CCAGCCTTCTGACCTCTTTCC	TCTTCGATTTGTTTTGCATCCA
GSTM1	TCTTGACCAGTACCACATTTTTGAG	TCGAAAATATAGGTGTTGAGAGGTAGTG
ERα	GGCACATGAGTAACAAAGGCA	GGCATGAAGACGATGAGCAT
ERβ	CTCCTTTAGCGACCCATTGC	CTCCCACTAAGCTTCCTCTTCAGT
PGR	TCACAACGCTTCTATCAACTTACAAA	GGCAGCAATAACTTCAGACATCA
β-Actin	GCCAACCGTGAAAAGATGAC	ACCCTCATAGATGGGCACAG

Primers were designed using Primer Express® software across exon boundary for the following genes: 17βHSD7- 17β hydroxyl steroid dehydrogenase 7;CYP1A1-Cytochrome P450 1A1; CYP1B1-Cytochrome P450 1B1; COMT- Catechol-O-methyl transferase; GSTA1- Glutathione-S-transferase A1; GSTM1-Glutathione-S-transferase M1; ERα-Estrogen receptor α; ERβ-Estogen receptor β; PGR- Progesterone receptor.