TABLE 1.
Quantification of DNA damage parameters in superoxide-treated HSCs
DNA damage |
|||
---|---|---|---|
Tail length (µm) | % DNA in tail | Tail moment (µm) | |
qHSCs | |||
Control | 28.3 ± 6.0 | 42.9 ± 5.7 | 18.1 ± 5.2 |
Superoxide | 35.3 ± 4.3 | 45.9 ± 6.8 | 17.6 ± 4.4 |
aHSCs | |||
Control | 6.7 ± 1.1 | 2.5 ± 1.1 | 3.8 ± 1.2 |
Superoxide | 32.0 ± 3.3* | 50.2 ± 5.8* | 19.6 ± 4.0* |
The cells were incubated with 1 mM hypoxanthine (Control) or with hypoxanthine and 2 mU/ ml xanthine oxidase (Superoxide) for 24 h. Comet assay was performed to assess DNA damage parameters.
P<0.001 versus control.