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Substrate-dependent effects of benzoquinone on mutant topoisomerase IIα-DNA binding. Assays employed a negatively supercoiled circular plasmid (shaded bars), a linear plasmid (open bars), or a duplex 50-mer oligonucleotide (filled bars) as the substrate. Enzymes were incubated with 100 μM benzoquinone for 5 min prior to the addition of DNA. DNA binding in the absence of benzoquinone was set to 100%. Error bars represent the standard deviation of at least three independent experiments.
