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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: J Cell Physiol. 2010 Jul;224(1):112–119. doi: 10.1002/jcp.22099

Figure 6. JNK and c-Jun activation are involved in vitamin k1 plus Sorafenib induced apoptosis in PL5 cells.

Figure 6

A. PL5 cells were treated with 50 µM vitamin k1 and 2.5 µM Sorafenib for 36 hours and levels of phospho-JNK and its key substrate phospho-c-Jun were analyzed by Western blot from whole-cell lysates. B. PL5 cells were treated with 50 µM vitamin k1 and 2.5 µM Sorafenib for 36 hours in the presence or absence of JNK inhibitor. After treatment, cells were lysed and 20 µg proteins were separated on SDS-PAGE gel or the percentage of apoptotic cells were determined by flow cytometry (C).