Figure 3. Protectin D1/neuroprotectin PD1 biosynthesis.
(A) DHA is converted by ALOX15 to the pivotal intermediate 17S-H(p)DHA that serves as a substrate for further enzymatic transformation to PD1/NPD1 via a 16(17) epoxide intermediate. This route of synthesis is distinct from the formation of the double dioxygenation product that does not share the same bioactions as PD1/NPD1. (B) Lipid extracts were obtained from inflamed murine lung and taken to LC-MS/MS that uncovered PD1/NPD1. Mass spectra revealing the characteristic fragmentation ions for PD1/NPD1.