A longitudinal assessment of H2O2 production by circulating monocytes (left panel) and lymphocytes (right panel) obtained serially from the same animals at baseline (wk 0), prior to treatment, and weekly during either ALDOST or ALDO+Spi. H2O2 production by flow cytometry, expressed as mean channel brightness, was significantly increased at wks 2, 3 and 4 of ALDOST. Spi co-treatment either prevented (monocytes) or attenuated (lymphocytes) this rise in H2O2 production in these cells. *p<0.05 vs. wk 0; †p<0.05 vs. Spi; mean±SD.