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. Author manuscript; available in PMC: 2010 Jul 2.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Sep 5;808(2):141–152. doi: 10.1016/j.jchromb.2004.04.030

Fig. 3.

Fig. 3

(A) Total ion chromatogram (TIC) for 0.5 mg/mL Doxorubicin in 40% (v/v) acetonitrile and 60% 5 mM ammonium acetate, pH 3.5. The LC–MS/MS was operated in positive ion mode using the turbo-spray ionization source. (A): A prominent peak for DXR was observed at m/z 544 [M + H]+ and selected for fragmentation. (B) Product ion chromatogram (XIC); fragmentation of the m/z 544 DXR ion produced prominent fragments of m/z 361 and 321. The m/z 544/361 ion-pair was selected and fragmentation conditions were further optimized for production of this product ion.