. Author manuscript; available in PMC: 2010 Jul 2.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Sep 5;808(2):141–152. doi: 10.1016/j.jchromb.2004.04.030

Table 5.

Effect of matrix on Doxorubicin analysis

Tissue	Nominal concentration (nM)	Peak area (CV%)			P-value
Tissue	Nominal concentration (nM)	Sample 1	Sample 2	Sample 3	P-value
Plasma	30	1985 (3.0)	1870 (11.0)	2090 (6)	0.239
Brain	65	7153 (7.8)	6853 (2.0)	6620 (2.0)	0.276
Liver	250	39767 (5.9)	33567 (7.8)	36600 (16)	0.236
Spleen	55	3740 (6.1)	3810 (4.1)	2290 (12.6)	0.237
Heart	65	5813 (2.3)	6120 (0.3)	5797 (4.3)	0.147
Lung	65	8087 (2.8)	8023 (2.4)	7767 (3.7)	0.302

Plasma and tissue samples from three different animals were spiked with DXR and extracted in triplicate. The peak area for DXR was compared and the coefficient of variation was determined; P ≤ 0.05 was considered an indication of significant change.