Table 9.

Metabolite identification after treatment of rats with SSL–DXR

Tissue	DXR-ol	DXR-one	DXR-olone
Plasma	+	+	−
Tumor	+	+	−
Brain	−	−	+
Liver	+	+	−
Spleen	+	+	+
Lung	+	+	−
Heart	+	+	−

Rats were treated intravenously with 5.67 mg/kg SSL–DXR, and plasma and tissues samples were harvested. The presence of DXR metabolites was determined by LC–MS/MS. Metabolites were considered present (+) if their peak height was greater than three times their background signal.

No evidence of 7-DXR-one or 7-DXR-olone was observed.