Table 1.
Rate and Equilibrium Constants For Inhibition and Turnover of Diaroyl Phosphates by β-Lactamases
| ko × 106 (s−1) | P99 | TEM | |||||
|---|---|---|---|---|---|---|---|
| ki (s−1mM−1) | k2 × 103 (s−1) | K1 (μM) | ki (s−1mM−1) | k2 × 103 (s−1) | Ki (μM) | ||
| 5 | 0.78 | 7 ± 1 | 9.3 ± 0.2 | 1.33a | 0.5 ± 0.1 | 38 ± 3 | 76a |
| 6 | 0.31 | 9 ± 1 | 1.28 ± 0.01 | 0.147a | 6.1 ± 0.1 | 1.98 ± 0.04 | 0.32a |
| 7 | 4.2 | 14 ± 1 | 58 ± 5 | 4.21a | 19.5 ± 3.0 | 39.5 ± 3.0 | 2.0a |
| 8 | 52 | 6 ± 1 | 65 ± 8 | 12.1a | 0.65b | 124 ± 5 | 190 ± 20 |
| 9 | 1.31 | 870 ± 20 | 27 ± 1 | 0.032a | 296b | 23.7 ± 1.0 | 0.08 ± 0.01 |
| 10 | 1.47 | 1810 ± 360 | 28 ± 1 | 0.016a | 40 ± 5 | 12.6 ± 0.1 | 0.31 |
| 11 | 54.1 | 69600b | 211 ± 6 | (3.0 ± 0.2) × 10−3 | 53b | 79 ± 2 | 1.47 ± 0.06 |
| 12 | 285 | 265b | 354 ± 26 | 1.3 ± 0.3 | 1.8b | 74 ± 1 | 41 ± 9 |
a
Calculated from Ki = k2/ki
b
Calculated from ki = k2/Ki