Expression of PMCA2, synapsin IIa and syntaxin 1B mRNA in the lumbar spinal cord of C57Bl/6 mice during the course of MOG-induced EAE. (A) Clinical course of the disease. Score 0, no symptoms; 1, flaccid tail; 2, one hindlimb paralysis; 3, paralysis of both hindlimbs. (B) PMCA2 transcript levels assessed by real-time PCR. C stands for control, animals immunized only with adjuvant. (C) Synapsin IIa transcript levels assessed by real-time PCR. (D) Syntaxin 1B transcript levels assessed by real-time PCR. **P < 0.01; *P < 0.05, compared with control. Note that PMCA2, synapsin IIa and Syntaxin 1B transcript levels are significantly reduced at onset of symptoms, similar to that observed in acute EAE in the rat.