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. 2010 Jun 17;29(1):76. doi: 10.1186/1756-9966-29-76

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Copyright ©2010 Liu et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Induction of apoptosis on B16-F10 cells by CPT-TMC in vitro. Cellular apoptosis was verified by flow cytometric analysis. B16-F10 Cells were treated with (a) media-only, (b) TMC, (c) CPT, or (d) CPT-TMC, respectively. It is clear that the number of apoptotic cells in CPT-TMC and CPT treated group was significantly higher compared with other two groups. The apoptotic rate showed 62% in CPT-TMC-treated group versus 57.1% in CPT-treated group, 10% in TMC-treated group and 3.9% in media-only-treated group.