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. Author manuscript; available in PMC: 2010 Jul 2.
Published in final edited form as: Gastroenterology. 2009 Feb 6;136(5):1772–1782. doi: 10.1053/j.gastro.2009.01.070

Figure 2.

Figure 2

Effect of inhibition of HSP70 expression on lysosomal integrity. (A) Inhibition of HSP70 expression by quercetin (Q) in both MiaPaCa-2 and Panc-1 pancreatic cancer cell lines leads to decreased Lysotracker green uptake at 12 hours, suggesting lysosomal permeabilization. (B) Inhibition of HSP70 expression by triptolide (T) also leads to decreased Lysotracker green uptake at 12 hours, suggesting lysosomal permeabilization. (C) Inhibition of HSP70 expression with 2 different siRNA sequences, siRNA1 and 2, in MiaPaCa-2 leads to lysosomal permeabilization. (D) Inhibition of HSP70 expression by siRNA1 leads to lysosomal permeabilization in Panc-1. (E) Inhibition of HSP70 expression in colon cancer cell line HT29 by triptolide and quercetin induced a decreased Lysotracker green uptake. Data are expressed as a percentage of control. Cells transfected with nonsilencing siRNA served as controls in siRNA experiments. Data are expressed as the mean ± SEM of 3 independent experiments. *P < .05 (t test) as compared with controls.