TABLE 2.
Gene expression analyses by quantitative real-time PCR
| 3 weeks | 8 weeks | 20 weeks | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Gene | P value | FDR | Fold-change | P value | FDR | Fold-change | P value | FDR | Fold-change |
| Cyp1b1 | 2.16E-03 | 4.33E-03 | 22.58 | 2.16E-03 | 2.89E-03 | 22.58 | 2.16E-03 | 3.46E-03 | 19.05 |
| Cry1 | 2.16E-03 | 4.33E-03 | 4.30 | 2.16E-03 | 2.89E-03 | 2.79 | 2.16E-03 | 3.46E-03 | 2.94 |
| Cbr3 | 2.16E-03 | 4.33E-03 | 3.56 | 2.16E-03 | 2.89E-03 | 2.55 | 2.16E-03 | 3.46E-03 | 2.79 |
| Ces3 | 6.49E-02 | 6.49E-02 | 2.38 | 8.66E-03 | 9.89E-03 | 1.68 | 4.11E-02 | 4.70E-02 | 1.57 |
| Tef | 2.16E-03 | 4.33E-03 | 2.53 | 6.49E-02 | 6.49E-02 | 1.54 | 4.33E-03 | 5.77E-03 | 1.87 |
| Ugt1a6a | 6.49E-02 | 6.49E-02 | 1.71 | 2.16E-03 | 2.89E-03 | 2.58 | 6.49E-02 | 6.49E-02 | 1.46 |
| Col3a1 | 1.52E-02 | 2.42E-02 | 0.54 | 2.16E-03 | 2.89E-03 | 0.30 | 2.16E-03 | 3.46E-03 | 0.38 |
| Hdc | 6.49E-02 | 6.49E-02 | 0.91 | 2.16E-03 | 2.89E-03 | 0.43 | 2.16E-03 | 3.46E-03 | 0.43 |
Total RNA was assayed by RT-PCR. Samples were analyzed in quadruplicate, and the resulting data were expressed as the average cycle threshold (Ct). The housekeeping gene Hprt1 was used for data normalization (ΔCt). Comparisons between control and smoke-exposed samples for statistical significance were determined using the Mann-Whitney test (P-value) and the step-up method of Benjamini & Hochberg (False Discovery Rate (FDR)). Fold-change was calculated using the Comparative Ct method (ΔΔCt). Genes induced or repressed following smoke exposure are indicated by a fold-change >1 or <1, respectively.