Fig. 4.

Dispersion of groESL-lacO₁₂₀ mRNA. a, Spatial distribution profiles of the 6.3-kb groESL-lacO₁₂₀ mRNA distribution profiles in a 3-μm virtual cell calculated with Eq.[6] (Supplementary Information), assuming that groESL-lacO₁₂₀ mRNA is freely diffusible and either ribosome-free (blue) or saturated with ribosomes (red). The dotted line delineates the source of mRNA (site of transcription). b, RNA-FISH image of lacO-Cy3 hybridized-groESL-lacO₁₂₀ mRNAs in CJW2966 cells after 15 min at 42°C. c, Representative lacO-Cy3 hybridized-groESL-lacO₁₂₀ mRNA intensity profiles of individual, heat-shocked CJW2966 cells. The blue dots are the experimental data and the black line is the best fit using the two-source (right) or one-source (left) model (see Supplementary Information). The red dashed line corresponds to the background fluorescence. d, Histogram of FWHM values of groESL-lacO₁₂₀ mRNA from fluorescence intensity profiles of CJW2966 cells grown at 30°C (blue) or after heat shock (42°C for 15 min; red).