Table 1. Surface and intracellular (ic) characterization of the expanded progeny (Mean, +/- SD).
Surface and intracellular phenotyping, PMA/ionomycin induced cytokine secretion was performed as described.(1-3)
| Variable | IL2 Only | IL2 + IL7 | p-value* |
|---|---|---|---|
| Day 12-14 Mean-fold expansion | 48 +/- 21 | 165 +/- 123 | 0.04 |
| %Viable T Lymphocytes among CD45+ events | 46 +/- 15 | 71 +/- 10 | 0.002 |
| %CD4+ | 60 +/- 20 | 65 +/- 16 | 0.26 |
| %CD8+ | 52 +/- 26 | 45 +/- 20 | 0.15 |
| %CD4+/CD8+ | 6 +/- 5 | 3 +/- 3 | 0.43 |
| ic BCL-2/CD3+ (MFI) | 82 +/- 19 | 82 +/- 16 | 0.97 |
| % ic Ki67+/CD3+ “proliferating” | 50 +/- 14 | 65 +/- 11 | 0.003 |
| %ic Ki67+/CD4+ “proliferating” | 50 +/- 15 | 62 +/- 10 | 0.03 |
| %ic Ki67+/CD8+ “proliferating” | 52 +/- 12 | 68 +/- 11 | 0.001 |
| % ic Activated Caspase 3+/CD3+ “apoptotic” | 8 +/- 3 | 4 +/- 2 | 0.011 |
| % ic Activated Caspase 3+/CD4+ “apoptotic” | 7 +/- 3 | 4 +/- 2 | 0.02 |
| % ic Activated Caspase 3+/CD8+ “apoptotic” | 8 +/- 5 | 4 +/- 2 | 0.03 |
| %CD25+/CD3+ | 62 +/- 14 | 63 +/- 17 | 0.95 |
| %CD25+/CD45RO+ | 43 +/- 19 | 25 +/- 28 | 0.08 |
| %CD28+/CD3+ | 89 +/- 5 | 95 +/- 3 | 0.02 |
| %CDRA+/RO- “naïve” | 45 +/- 30 | 78 +/- 28 | 0.06 |
| %CDRA-/RO+ “memory” | 10 +/- 4 | 18 +/- 27 | 0.65 |
| %CDRA+/CD62L+ “phenotypically naive” | 73 +/- 14 | 90 +/- 5 | 0.03 |
| %CDRA+/CD27+/CD8+ “naïve CD8+” | 79 +/- 7 | 90 +/- 4 | 0.003 |
| %CDRA-/CD27+/CD8+ “central memory” | 13 +/- 6 | 4 +/- 2 | 0.002 |
| %CDRA+/CD27-/CD8+ “effector CTL” | 7 +/- 6 | 6 +/- 4 | 0.26 |
| %CD57+/CD28-/CD8+ “effector CTL” | 0 +/- 0 | 0 +/- 0 | 0.35 |
| %HLA-DR+/CD3+ “activated” | 46 +/- 12 | 44 +/- 11 | 0.53 |
| %HLA-DR+/CD4+ “activated” | 45 +/- 12 | 37 +/- 11 | 0.1 |
| %HLA-DR+/CD8+ “activated” | 45 +/- 16 | 53 +/- 15 | 0.003 |
| %NKG2D+/CD3+ | 38 +/- 23 | 27 +/- 20 | 0.03 |
| %NKG2D+/CD137-/CD8+ “resting” | 73 +/- 17 | 62 +/- 26 | 0.05 |
| %NKG2D-/CD137+/CD8+ “activated” | 1 +/- 2 | 0 +/- 0 | 0.32 |
| %NKG2D-/CD137-/CD8+ “anergic” | 24 +/- 16 | 37 +/- 26 | 0.07 |
| %NKG2D+/CD137+/CD8+ “cytotoxic” | 2 +/- 3 | 1 +/- 1 | 0.24 |
| % ic GranzymeA+/CD8+ | 44 +/- 26 | 44 +/- 23 | 0.95 |
| % ic GranzymeB+/CD8+ | 41 +/- 35 | 35 +/- 25 | 0.26 |
| % ic Perforin+/CD8+ | 6 +/- 4 | 4 +/- 2 | 0.10 |
| % ic INFγ+/CD3+ | 20 +/- 8 | 19 +/- 10 | 0.64 |
| % ic TNFα +/CD3+ | 50 +/- 20 | 55 +/- 27 | 0.65 |
| % ic IL2+/CD4+ | 79 +/- 9 | 54 +/- 36 | 0.41 |
| % ic IL4+/CD3+ | 2 +/- 2 | 2 +/- 2 | 0.55 |
| % OX40+/CD4+ | 83 +/- 24 | 78 +/- 21 | 0.79 |
*
2-tailed, paired Student t-test
MFI: Mean Fluorescence Index