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. Author manuscript; available in PMC: 2010 Jul 3.
Published in final edited form as: J Immunol. 2007 Oct 15;179(8):5181–5190. doi: 10.4049/jimmunol.179.8.5181

Figure 3.

Figure 3

Counter-inhibition assay used to identify canonical Ab of switched specificity. A, Binding of biotin-labeled Ab 3A4 to anti-Id E4 is inhibited by canonical E4+ Id in serum. If serum Id binds hapten, inhibition is reversed. Note, 3A4 does not bind Ars-Tyr or Sulf-Tyr. B, Representative results for two sera, one of which contains E4+ Id that binds more strongly to Sulf-Tyr than Ars-Tyr (switched specificity, representative of group #5 Table IV), and the other of which behaves conversely (not switched, representative of control group Table IV).