Table II.

Amino acid replacements (somatic mutations) in V genes of Sulf-switch mutant canonical mAb.

S2-2		S3-4		S10-16
VH	Vκ	VH	Vk	VH	Vκ
Q5 -> H	D1 -> E	Q5 -> K	M4 -> L	A9 -> T	I2 -> T
A14 -> S	S22 -> T	K19 -> Q	T8 -> P	Y27 -> F	A25 -> S
T28 -> P	D28 -> G	T28 -> S	V19 -> I	T28 -> S	K39 -> R
F29 -> V	S30 -> N	S31 -> N	A25 -> S	S31 -> T	D70 -> H
S31 -> N	V44 -> M	N35 -> S	S30 -> N	I34 -> V	S76 -> N
Y32 -> F	K45 -> R	G49 -> A	N31 -> S	N35 -> V	I83 -> V
I34 -> V	H55 -> Q	N52 -> S	K45 -> R	E46 -> Q	N92 -> Y
N35 -> V	G64 -> A	Y57 -> F	G68 -> K	N52 -> S
G42 -> S	Y71 -> F	K59 -> S	S76 -> N	G54 -> E
N52 -> S	Q80 -> P	G66 -> D	N92 -> D	N55 -> G
Y57 -> F	I83 -> F	K67 -> Q	T93 -> A	Y57 -> F
K59 -> E	N92 -> S	V72 -> G		K59 -> E
G66 -> V	T93 -> A	K74 -> R		E62 -> A
V72 -> I		M81 -> L		K63 -> R
K74 -> Q		A97 -> V		K65 -> T
M81 -> L		F108 -> Y		V72 -> M
V93 -> I				K74 -> R
N100 -> T
Q113 -> R

Bold values represent critical residues conferring change in specificity from Ars to Sulf