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. Author manuscript; available in PMC: 2011 Jul 7.
Published in final edited form as: J Am Chem Soc. 2010 Jul 7;132(26):9122–9128. doi: 10.1021/ja102519v

Figure 2.

Figure 2

LC-MS-MS analysis of the metabolite A-74528. Triple quadrupole LC-MS multiple reaction monitoring detection of A-74528 in extracts from (A) wild-type Streptomyces. sp SANK 61196, (B) wild-type Streptomyces griseus, (C) Streptomyces lividans K4-114/pKZ11, (D) mutant Streptomyces sp. SANK 61196ΔKS, (E) Streptomyces lividans K4-114/pKZ11, (F) Streptomyces lividans K4-114/pKZ12, (G) Streptomyces lividans K4-114/pKZ13, and (H) Streptomyces lividans K4-114/pKZ14. The peaks shown correspond to a metabolite with a parent ion of 561 (m/z) and a fragment ion of 417 (presumably corresponding to the loss of the lactone moiety of A-74528)