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. Author manuscript; available in PMC: 2011 Jun 30.
Published in final edited form as: J Am Chem Soc. 2010 Jun 30;132(25):8610–8617. doi: 10.1021/ja910844n

Figure 2.

Figure 2

(a) Sedimentation coefficient distributions for various cyt cb562 constructs at 5 μM monomeric concentration and equimolar Zn2+ where indicated. For MBPC-1 and RIDC-1, the Zn-induced tetrameric species are fully populated at >1 mM and >20 μM protein and Zn, respectively. At 5 μM protein and Zn, MBPC-1 is still predominantly monomeric (Smax= 1.8), while RIDC-1 is a mixture of dimeric (Smax= 2.8) and tetrameric (Smax= 4.5) forms. (b) Sedimentation equilibrium profile for 2.5 μM C96RIDC-12 obtained at 20000 rpm (see Figure S2 for other concentrations and speeds). The sedimentation data are equally well described by a dimer-tetramer equilibrium (Kd = 52 nM) or a tetramer-only model, which suggests the tetramer dissociation constant to be <100 nM.