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. Author manuscript; available in PMC: 2011 Jun 30.
Published in final edited form as: J Am Chem Soc. 2010 Jun 30;132(25):8610–8617. doi: 10.1021/ja910844n

Figure 7.

Figure 7

(a) Cu2+ and (b) Ni2+ binding isotherms for Fura-2–C96RIDC-14 competition experiments; corresponding changes in the Fura-2 absorbance spectrum are shown in the inset. The samples contained 7.5 μM C96RIDC-14 and 11 μM Fura-2. The tick marks shown on the top x-axis correspond to theoretical endpoints for titration if C96RIDC-14 bound to one, two, three or four equivalents of metal. The fits obtained using DynaFit are shown for the following different models: solid line, two consecutive binding equilibria (1+1); dashed line, single binding equilibrium (2). Equilibrium constants obtained with these different models are listed in Table 1.