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. 2010 Mar 2;38(12):4133–4142. doi: 10.1093/nar/gkq067

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Influence of helix (de-)stabilizing effects: salt and force. (a) Binding curves recorded at 0.5 pN and [RPA]=40 nM for [NaCl]=30, 60, 90 and 120 mM. The salt concentration has a marked effect on the binding kinetics. High-salt traces are characterized by a salt-dependent lag time and slower extension rates. (b) Extension rates at varying [hRPA] concentrations. The reaction rates are linearly dependent on [hRPA]. (c) Binding curve at low salt ([NaCl]=30 mM) followed by dissociation at high salt ([NaCl]=120 mM) after removal of hRPA. (d) Association and dissociation rates on a log–log scale. (e) Dissociation curves measured at varying stretching force. (f) Association and dissociation rates as function of force reveal a strong force dependence of the reaction kinetics.