Table 1.

Genes and primers for pyrosequencing

Gene	Primer	Sequence (5′–3′)	Amplicon length (bp)	Chromosomal localization (bp)	Number of CpGs	Reference
H19	Outer forward	TTTTTGGTAGGTATAGAGTT	231	Chromosome 11 1 977 647-1 977 878	4	El-Maarri et al. (30)
	Outer reverse	AAACCATAACACTAAAACCC
	Nested forward	TGTATAGTATATGGGTATTTTTGGAGGTTT
	Nested reverse*	TCCTATAAATATCCTATTCCCAAATAACC
	Sequencing	TGGTTGTAGTTGTGGAAT
MEG3	Forward	GATTTTTTTTATATATTGTGTTTG	220	Chromosome 14 100 361 907-100 362 129	3	Zechner et al. (35)
	Reverse*	CTCATTTCTCTAAAAATAATTAACC
	Sequencing	GTGTTTGAATTTATTTTGTTTGG
LIT1	Forward	AATTAGTAGGTGGGGGG	122	Chromosome 11 2 677 751-2 677 873	2	Mackay et al. (31)
	Reverse*	CTAAAAAACTCCCTAAAAATC
	Sequencing	GGGGGTAGTYGGAG
NESP55	Outer forward	TTTTTTATTTTATAGGGTGTATTT	343	Chromosome 20 56 848 506-56 848 849	3	El-Maarri et al. (30)
	Outer reverse	AAAATAAAATACTTAAACACCAC
	Nested forward	TTTTTGTAGAGTTAGAGGGTAGGT
	Nested reverse*	AAAAAAAACAACTCAAAATCTACC
	Sequencing	GTGTTTAAGAGGATGGAT
PEG3	Forward	GGTGTAGAAGTTTGGGTAGTTG	153	Chromosome 19 62 043 756-62 043 909	4	Mackay et al. (31)
	Reverse*	CTCACCTCACCTCAATACTAC
	Sequencing	TGTTTATTTTGGGTTGGT
SNRPN	Forward*	AGGGAGTTGGGATTTTTGTATT	237	Chromosome 15 22 751 105-22 751 342		White et al. (32)
	Reverse	CCCAAACTATCTCTTAAAAAAAAC			2 × 3 = 6
	Sequencing 1	ACACAACTAACCTTACCC			3
	Sequencing 2	CCAACCTACCTCTAC			3
OCT4	Forward	AAGTTTTTGTGGGGGATTTGTAT	185	Chromosome 6 31 246 461-31 246 646	2	Deb-Rinker et al. (33)
	Reverse*	CCACCCACTAACCTTAACCTCTA
	Sequencing	TGAGGTTTTGGAGGG
APC	Forward*	GGTTAGGGTTAGGTAGGTTGT	193	Chromosome 5 112 101 274-112 101 467	7	Schatz et al. (34)
	Reverse	ACTACACCAATACAACCACATATC
	Sequencing	CCACACCCAACCAA

This table provides the primer sequences for bisulfite pyrosequencing of our studied genes, the length and chromosomal localization (according to ensembl 54, May 2009) of the amplified segments, and the number of CpG sites in the amplicons. Biotinylated primers are indicated by star symbols.