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. 2010 Mar 9;38(12):3909–3922. doi: 10.1093/nar/gkq132

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — L1 and SpORF2 expression in normal human cells results in DNA damage. (A) Normal human fibroblasts transiently transfected with the empty vector or vectors expressing L1 or SpORF2 were analyzed for 53BP1 foci formation 21 h post-transfection. 53BP1 is one of the characterized responders recruited to the sites of DNA DSBs and it is commonly used as a marker for DNA DSBs. Treatment with 1.85 mM of H₂O₂ for 10 min was used as positive control. Overlay of Hoechst and antip53BP antibodies is shown. Arrows indicate cells with distinct staining foci. (B) Quantitation of the 53BP1 foci positive cells as a percent of the total cells (mean ± SD). Asterisks indicate significant difference determined by paired t-test with P = 0.024, 0.014, 0.001 for L1, spORF2 and H₂O₂, respectively. (C) Quantitative assessment of the average number of 53BP1 foci per cell (mean ± SD). Asterisks indicate significant difference determined by paired t-test with P = 0.003, 0.017 and 0.006 for L1, spORF2 and H₂O₂, respectively.