Figure 4.
Effect of ATM inhibitor on foci formation by UV micro-irradiation. Exponentially growing normal human diploid cells were incubated for 72 h with 10 µM BrdU. Cells were treated with 40 µM of KU55933 for 30 min before exposure to UVC (UV+KU). They were washed with PBS and exposed to 30 J/m2 of UVC through 5-µm pore size membrane (UV and UV+KU). Then, they were incubated for 30 min, fixed with 4% formaldehyde permeabilized with 0.5% Triton X-100, and incubated with mouse anti-phosphorylated histone H2AX at serine 139. (No UV) Mock irradiated. Bar indicates 2 µm.