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. 2010 Apr 20;38(12):e133. doi: 10.1093/nar/gkq233

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — The novel monoclonal antibody is specific for 4-OHEN-DNA adducts. (A) The antibody shows high binding to 4-OHEN-ssDNA but undetectable or minimal binding to undamaged ssDNA. Different dilutions of the antibody were tested for binding to immobilized denatured antigens (50 ng/well) using a direct ELISA. (B) The antibody binds to 4-OHEN–DNA adducts but not to other types of DNA damage. Competitive inhibition of the antibody (1/10 000) binding to immobilized 4-OHEN-ssDNA (40 ng/well) by various competitors was performed using a competitive ELISA. (C) The antibody does not bind to a DNA adduct derived from endogenous estrogen (17β-estradiol). Competitive inhibition of the antibody (1/3000) binding to immobilized 4-OHEN-ssDNA (5 ng/well) by various competitors was performed using a competitive ELISA. oligo, oligonucleotide; dG-N²-6β-E₂-oligo, oligonucleotide containing a deoxyguanosine-N²-6β-estradiol. Each point shows the mean (±SD) of three independent experiments.