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FISH validation of low frequency amplifications in paediatric high grade glioma. Specific probes for MYCN, PIK3CA, PDGFRA, SKP2, PDGFRB, MYC, CDK4, MDM2, and IGF1R were labelled with Cy5 (red) and co-hybridised to interphase nuclei on FFPE specimens with chromosome-specific control probes labelled with fluoroscein.
