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. Author manuscript; available in PMC: 2011 Jan 26.

Published in final edited form as: Biochemistry. 2010 Jan 26;49(3):601–610. doi: 10.1021/bi901579y

Progress curves for p51^W401A binding to efavirenz monitored by tryptophan fluorescence at 5 °C. λ_ex = 295 nm, λ_em = 340 nm. 2.5 μM p51^W401A; () 5 μM, () 8 μM, () 11 μM, and (—) 14 μM efavirenz. Data acquired at 30 s intervals for first 4–5 h, then at 5 min intervals. Data were fit to eq 2a to obtain k_obs.

Inline graphic — Progress curves for p51^W401A binding to efavirenz monitored by tryptophan fluorescence at 5 °C. λ_ex = 295 nm, λ_em = 340 nm. 2.5 μM p51^W401A; () 5 μM, () 8 μM, () 11 μM, and (—) 14 μM efavirenz. Data acquired at 30 s intervals for first 4–5 h, then at 5 min intervals. Data were fit to eq 2a to obtain k_obs.