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. Author manuscript; available in PMC: 2011 Jan 26.

Published in final edited form as: Biochemistry. 2010 Jan 26;49(3):601–610. doi: 10.1021/bi901579y

Dissociation of () p51^W401A—EFV and () p66^W401A—EFV complexes monitored by tryptophan fluorescence at 5 °C. λ_ex = 295 nm, λ_em = 340 nm. Black curves are fits to eq 2b. Data acquired at 30 s intervals for first 4–5 h, then at 5 min intervals.

Inline graphic — Dissociation of () p51^W401A—EFV and () p66^W401A—EFV complexes monitored by tryptophan fluorescence at 5 °C. λ_ex = 295 nm, λ_em = 340 nm. Black curves are fits to eq 2b. Data acquired at 30 s intervals for first 4–5 h, then at 5 min intervals.